by Chapman and Hall; [distributed in the U.S.A. by Halsted Press, New York] in London .
Written in English
|Other titles||Histochemical journal.|
|Statement||Edited by P. J. Stoward. Foreword by A. G. E. Pearse.|
|Contributions||Stoward, P. J., ed.|
|LC Classifications||QH613 .F58|
|The Physical Object|
|Pagination||xiii, 201 p.|
|Number of Pages||201|
|LC Control Number||73006356|
The chemical and physical principles of fixation, staining, and histochemistry in one volume! Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue Cited by: Fixation in Histochemistry Dennis Jones (auth.), P. J. Stoward (eds.) by by Professor Professor A. A. G. G. Everson Pearse Pearse The tide of of the Symposium, at at which the the papers which compromise this volume were presented, is ofthe utmost importance. Genre/Form: Conference papers and proceedings Collected Work Congresses: Additional Physical Format: Online version: Fixation in histochemistry. London, Chapman and Hall; [distributed in the U.S.A. by Halsted Press, New York] Part of the Methods in Molecular Biology book series (MIMB, volume ) Abstract. A number of techniques have been developed to use chemical, immunological, and molecular biology assays in histological material. Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy. In: Hewitson T., Darby I.
Fixation profoundly affects histological and immunohistochemical staining, technicians, pathologists and research workers must therefore decide on the most appropriate method. aspects to consider are temperature, size of the storage container, volume ratio, salt concentration, ph and incubation time. Scion publishing, £ The purpose of this well-established book, as stated in the preface, is ‘to teach the chemical, physical and biological principles of fixation, staining and histochemistry’. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. conventional histochemistry, to demonstrate insoluble compounds the perfusion fixation can be used and the tissues from various or gans were taken out, cut into small pieces (1 x 1 x 1 mm), soaked.
The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as . Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. Fixation 2. Formic acid treatment 3. Tissue processing 4. Histological staining 5. PrP immunolabelling & antibodies 6. Histology laboratory technical tips. 1. Fixation. Whole Brain: Brains are fixed in a 10 x volume (4 to 6 litres for bovine brain, 1 to 2 litres for ovine brain) of 10% formal saline or 10% neutral buffered formalin (Supplier. This fifth edition of Histological and Histochemical Methods continues to provide a clear and consistent introduction to the techniques, description and analysis of the chemical and physical principles of fixation, tissue processing, staining, enzyme location, immunohistochemistry and other key procedures.. The overall structure of the book remains unchanged, but the content has been heavily.